CCM3/PDCD10 Monoklonaler Antikörper

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• KD/KO Validated

CCM3/PDCD10 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC

Konjugation

Unkonjugiert

CloneNo.

1E5A8

Kat-Nr. : 66440-1-Ig

Synonyme

CCM3, PDCD10, programmed cell death 10, TFAR15

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Galerie der Validierungsdaten

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  WB analysis of U-251 using 66440-1-Ig

  WB result of CCM3/PDCD10 antibody (66440-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDCD10 transfected U-251 cells.

• 

  WB analysis of Jurkat using 66440-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66440-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 66440-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66440-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66440-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66440-1-Ig (PDCD10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66440-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66440-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Ramos-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66440-1-Ig bindet in WB, IHC CCM3/PDCD10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CCM3/PDCD10 fusion protein Ag0348
• Vollständiger Name: programmed cell death 10
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC002506
• Gene symbol: PDCD10
• Gene ID (NCBI): 11235
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development.