PDCD2L Monoklonaler Antikörper

PDCD2L Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G12C4

Kat-Nr. : 68249-1-Ig

Synonyme

PDCD2L, programmed cell death 2 like

---

• 

  WB analysis using 68249-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68249-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 68249-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDCD2L antibody (68249-1-Ig, Clone: 2G12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68249-1-Ig bindet in WB, IF/ICC, ELISA PDCD2L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDCD2L fusion protein Ag30807
• Vollständiger Name: programmed cell death 2-like
• Berechnetes Molekulargewicht: 358 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC006146
• Gene symbol: PDCD2L
• Gene ID (NCBI): 84306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDCD2L was aberrantly expressed in multiple types of human cancers, and associated with clinical stage and molecular subtype and PDCD2L could be served as a biomarker of CRC (PMID: 35216602).