PDE6B Polyklonaler Antikörper

PDE6B Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 22063-1-AP

Synonyme

CSNB3, GMP PDE beta, PDE6B, PDEB, rd1, RP40

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Galerie der Validierungsdaten

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  WB analysis of mouse eye tissue using 22063-1-AP

  WB results of PDE6B antibody (22063-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

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  WB analysis of mouse eye using 22063-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 22063-1-AP (PDE6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22063-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22063-1-AP (PDE6B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat eye using 22063-1-AP

  rat eye tissue were subjected to SDS PAGE followed by western blot with 22063-1-AP (PDE6B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 22063-1-AP

  IP Result of anti-PDE6B (IP:22063-1-AP, 4ug; Detection:22063-1-AP 1:1000) with Y79 cells lysate 2000ug.

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  IF Staining of mouse eye using 22063-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PDE6B antibody (22063-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, A549-Zellen, Ratten-Augengewebe
• Erfolgreiche IP: Y79-Zellen
• Erfolgreiche Detektion in IF: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

22063-1-AP bindet in WB, IP, IHC, IF, ELISA PDE6B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE6B fusion protein Ag17139
• Vollständiger Name: phosphodiesterase 6B, cGMP-specific, rod, beta
• Berechnetes Molekulargewicht: 854 aa, 98 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC000249
• Gene symbol: PDE6B
• Gene ID (NCBI): 5158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE6 is a cGMP-specific PDE family and presents multicomponent enzyme complexes. The rod PDE6 enzyme is comprised of two catalytic subunits (PDE6A and PDE6B) and it is expressed in human lungs (PMID:20979602). PDE6B(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta) is also named as PDEB and belongs to the cyclic nucleotide phosphodiesterase family. PDE6B also has a C-terminal CAAX motif for posttranslational processing involving lipidation, proteolysis, and carboxymethylation (PMID:8394243). Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40)(PMID:22334370) and congenital stationary night blindness autosomal dominant type 2 (CSNBAD2) (PMID:8075643).

Publikationen

Species	Application	Title
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB,IF	FEBS J; Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice; Authors - Xiao Li
zebrafish	WB	J Biol Chem; Pathogenic Mutations in Retinitis Pigmentosa 2 Predominantly Result in Loss of RP2 Protein Stability in Human and Zebrafish.; Authors - Fei Liu
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
mouse	WB	Dev Biol; Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis.; Authors - Jeremy R Egbert
mouse	WB, IHC	PLoS One; Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.; Authors - Poppy Datta