PDH E1 Alpha Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDH E1 Alpha Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

56

Rezensionen (3)

Kat-Nr. : 18068-1-AP

Synonyme

PDH E1 alpha, PDH E1α, PDHA1, PDHE1 A type I, pyruvate dehydrogenase E1 α subunit

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Galerie der Validierungsdaten

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  WB analysis using 18068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK293 cells using 18068-1-AP

  WB result of PDHA1 antibody (18068-1-AP, 1:1000) with si-control and si-PDHA1 transfected HEK293 cells.

• 

  WB analysis using 18068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18068-1-AP (PDH E1α antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 18068-1-AP

  IP Result of anti-PDH E1α (IP:18068-1-AP, 4ug; Detection:18068-1-AP 1:1000) with mouse kidney tissue lysate 4400ug.

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  IHC staining of human liver cancer using 18068-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18068-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDH E1 Alpha antibody (18068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Rattenhirngewebe, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 51 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

18068-1-AP bindet in WB, IP, IHC, IF, ELISA PDH E1 Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDH E1 Alpha fusion protein Ag12701
• Vollständiger Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 39-48 kDa
• GenBank-Zugangsnummer: BC002406
• Gene symbol: PDHA1
• Gene ID (NCBI): 5160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing with the molecular mass of 39-48 kDa. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Publikationen

Species	Application	Title
mouse	WB,IP	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors - ; KO Validated
rat	WB	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
human	WB	Cell Metab; Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer.; Authors - Shawn M Davidson ; KO Validated
human	WB	Mol Cell; Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.; Authors - Ji Zhang ; KD Validated
human	WB	Circ Res; (Pro)renin Receptor Inhibition Reprograms Hepatic Lipid Metabolism and Protects Mice from Diet-Induced Obesity and Hepatosteatosis.; Authors - Liwei Ren

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and got nice bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:18068-1-AP)

FH

David (Verified Customer) (09-03-2019)

Good detection with 30µg of protein and dilution at 1/1000

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Brain

PDH E1 Alpha Antibody Western Blot, validation (1/1000 dilution) in Brain (Cat no:18068-1-AP)

FH

Svitlana (Verified Customer) (11-02-2017)

Loading:per well 15 ug/ulGradient gel 4-12%Transfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hImaging:Odyssey CLx Imaging

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line).

PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line). (Cat no:18068-1-AP)