ERp72 Polyklonaler Antikörper

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• KD/KO Validated

ERp72 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Kat-Nr. : 14712-1-AP

Synonyme

Protein disulfide-isomerase A4, PDIA4, ERp-72, ERp70, ERp 72

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  WB analysis using 14712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14712-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14712-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14712-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14712-1-AP

  IP result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of human liver cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 14712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of PC-3 using 14712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HepG2 using 14712-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 31 publications below
• IHC: See 3 publications below
• IF: See 16 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14712-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA ERp72 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERp72 fusion protein Ag6431
• Vollständiger Name: protein disulfide isomerase family A, member 4
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC000425
• Gene symbol: ERp72
• Gene ID (NCBI): 9601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

Publikationen

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
human	IF	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
human	WB	J Thromb Haemost; SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia; Authors - Jiasheng Zhang
human	WB	Mol Syst Biol; Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics; Authors - Madison T Wright
		Elife; Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation.; Authors - Lars Plate
human	WB	Cell Chem Biol; Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.; Authors - Lars Plate