PDK4 Rekombinanter Antikörper

PDK4 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240551E4

Kat-Nr. : 83583-1-RR

Synonyme

PDHK4, EC:2.7.11.2, 240551E4, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

---

• 

  WB analysis of mouse kidney using 83583-1-RR

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 83583-1-RR (PDK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 83583-1-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83583-1-RR (PDK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 83583-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 83583-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 83583-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 83583-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 83583-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDK4 Recombinant antibody (83583-1-RR, Clone:240551E4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83583-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83583-1-RR against Human PDK4 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83583-1-RR bindet in WB, IHC, FC (Intra), ELISA PDK4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDK4 fusion protein Ag3629
• Vollständiger Name: pyruvate dehydrogenase kinase, isozyme 4
• Berechnetes Molekulargewicht: 411 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC040239
• Gene symbol: PDK4
• Gene ID (NCBI): 5166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).