PDLIM1/CLP36 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDLIM1/CLP36 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 11674-1-AP

Synonyme

CLIM1, CLP 36, CLP36, Elfin, hCLIM1, LIM domain protein CLP 36, PDLIM1, PDLIM1/CLP36, PDZ and LIM domain 1, PDZ and LIM domain protein 1

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Galerie der Validierungsdaten

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  WB analysis using 11674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11674-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ileum using 11674-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11674-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse heart using 11674-1-AP

  IP Result of anti-PDLIM1/CLP36 (IP:11674-1-AP, 3ug; Detection:11674-1-AP 1:600) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 11674-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 11674-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PDLIM1 antibody 11674-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of MCF-7 using 11674-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDLIM1/CLP36 antibody (11674-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 11674-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PDLIM1/CLP36 antibody (11674-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, humanes Ileumgewebe, humanes Lungengewebe, Mausherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Produktinformation

11674-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PDLIM1/CLP36 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDLIM1/CLP36 fusion protein Ag2274
• Vollständiger Name: PDZ and LIM domain 1
• Berechnetes Molekulargewicht: 329 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC000915
• Gene symbol: PDLIM1
• Gene ID (NCBI): 9124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)

Publikationen

Species	Application	Title
mouse	WB,IF	Autophagy; Autophagy is required for ectoplasmic specialization assembly in Sertoli cells.; Authors - Chao Liu ; KD Validated
mouse	WB	Autophagy; Autophagy regulates spermatid differentiation via degradation of PDLIM1.; Authors - Yongliang Shang
human	WB	EMBO Rep; Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB.; Authors - Yusha Wang
mouse	WB	Open Biol; Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.; Authors - Shiguo Li
human	WB,IF	Exp Cell Res; CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.; Authors - Miyazaki Kazufumi K ; KD Validated
human,rat	WB,IF	Neurosci Lett; CLP36 interacts with palladin in dorsal root ganglion neurons.; Authors - Hasegawa Tomohiko T

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

PDLIM1 shows up as a clear band at the correct molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

PDLIM1/CLP36 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:11674-1-AP)