PELP1 Polyklonaler Antikörper

PELP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30135-1-AP

Synonyme

HMX3, MNAR, P160, PELP1, Transcription factor HMX3

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Galerie der Validierungsdaten

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  WB analysis using 30135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30135-1-AP (PELP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 30135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 30135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30135-1-AP (PELP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 30135-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PELP1 antibody (30135-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30135-1-AP bindet in WB, IHC, IF, ELISA PELP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PELP1 fusion protein Ag32720
• Vollständiger Name: proline, glutamate and leucine rich protein 1
• Berechnetes Molekulargewicht: 1130 aa, 120 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC069058
• Gene symbol: PELP1
• Gene ID (NCBI): 27043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.