PEX14 Polyklonaler Antikörper

PEX14 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen

6

Kat-Nr. : CL594-10594

Synonyme

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL594-10594

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PEX14 antibody (CL594-10594) at dilution of 1:400, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

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  IF Staining of HeLa using CL594-10594

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PEX14 antibody (CL594-10594) at dilution of 1:400, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

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  FC experiment of HeLa using CL594-10594

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human PEX14 (CL594-10594) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 6 publications below

Produktinformation

CL594-10594 bindet in IF, FC (Intra) PEX14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PEX14 fusion protein Ag0932
• Vollständiger Name: peroxisomal biogenesis factor 14
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC006327
• Gene symbol: PEX14
• Gene ID (NCBI): 5195
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Publikationen

Species	Application	Title
rat	IF	Mol Neurobiol; Schwann Cell-Derived Exosomes Ameliorate Paclitaxel-Induced Peripheral Neuropathy Through the miR-21-Mediated PTEN Signaling Pathway; Authors - Min You
mouse	IF	Inflamm Res; GCH1 reduces LPS-induced alveolar macrophage polarization and inflammation by inhibition of ferroptosis; Authors - Yuhong Xiao
mouse	IF	J Neuroinflammation; TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy; Authors - Xuliang Huang
human,mouse,rat	IF	Am J Physiol Cell Physiol; OTUD6A in tubular epithelial cells mediates Angiotensin II-induced kidney injury by targeting STAT3; Authors - Xiaoyu Sun
	IF	Pharmaceuticals (Basel); A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/Ca2+ Signaling Pathway; Authors - Jingyun Liu
mouse	IF	J Ethnopharmacol; TMT quantitative proteomics and network pharmacology reveal the mechanism by which asiaticoside regulates the JAK2/STAT3 signaling pathway to inhibit peritoneal fibrosis; Authors - Jinyi Sun

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C