PEX5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PEX5 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 12545-1-AP

Synonyme

Cell and organelle markers, Peroxin 5, Peroxisome Marker, Peroxisome receptor 1, PEX5, PTS1 BP, PTS1 receptor, PTS1R, PXR1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12545-1-AP

  WB result of PEX5 antibody (12545-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX5 transfected HEK-293 cells.

• 

  WB analysis of human brain using 12545-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12545-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12545-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12545-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12545-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12545-1-AP

  IP Result of anti-PEX5 (IP:12545-1-AP, 4ug; Detection:12545-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human kidney using 12545-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12545-1-AP (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12545-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12545-1-AP (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 12545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12545-1-AP (PEX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12545-1-AP (PEX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12545-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12545-1-AP (PEX5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 12545-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug PEX5 antibody (12545-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen, humanes Nierengewebe, L02-Zellen, Maushirngewebe, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

12545-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PEX5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PEX5 fusion protein Ag3268
• Vollständiger Name: peroxisomal biogenesis factor 5
• Berechnetes Molekulargewicht: 631 aa, 70 kDa
• Beobachtetes Molekulargewicht: 68-80 kDa
• GenBank-Zugangsnummer: BC010621
• Gene symbol: PEX5
• Gene ID (NCBI): 5830
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder. This antibody recognizes endogenous PEX5, which migrates with an apparent molecular mass of 80 kDa (PMID: 7790377).

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; A tuberous sclerosis complex signalling node at the peroxisome regulates mTORC1 and autophagy in response to ROS.; Authors - Zhang Jiangwei J ; KD Validated
human	WB, IF	Nat Cell Biol; ATM functions at the peroxisome to induce pexophagy in response to ROS.; Authors - Jiangwei Zhang
human	WB,IF,IP	Autophagy; Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.; Authors - Michael S Dahabieh
human	WB	Hum Mol Genet; A novel type of rhizomelic chondrodysplasia punctata, RCDP5, is caused by loss of the PEX5 long isoform.; Authors - Tuva Barøy
		Biochim Biophys Acta Mol Cell Biol Lipids; Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes.; Authors - Zhihui Zhu
rat	WB	Eur J Pharmacol; PEX5 prevents cardiomyocyte hypertrophy via suppressing the redox-sensitive signaling pathways MAPKs and STAT3.; Authors - Minghui Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhihui (Verified Customer) (12-12-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T And mouse astrocytes

PEX5 Antibody Western Blot, validation (1:1000 dilution) in HEK293T And mouse astrocytes (Cat no:12545-1-AP)

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