PFKL Polyklonaler Antikörper

PFKL Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 15652-1-AP

Synonyme

PFK B, PFKL, Phosphofructokinase 1, phosphofructokinase, liver, Phosphohexokinase

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  WB analysis of HeLa using 15652-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15652-1-AP (PFKL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15652-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15652-1-AP (PFKL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15652-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15652-1-AP (PFKL antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

15652-1-AP bindet in WB, IHC, ELISA PFKL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PFKL fusion protein Ag8250
• Vollständiger Name: phosphofructokinase, liver
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC008964
• Gene symbol: PFKL
• Gene ID (NCBI): 5211
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFKL(6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK)(ATP:D-fructose-6-phosphate-1-phosphotransferase).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings,PFK exsits as a system of three of subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs(PMID: 2139864). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.; Authors - Shaoxun Xiang
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB	Cancer Res; Glucose catabolism in liver tumors induced by c-MYC can be sustained by various PKM1/PKM2 ratios and pyruvate kinase activities.; Authors - Andrés Méndez-Lucas
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong
mouse	WB	iScience; Phosphorylated YBX2 is stabilized to promote glycolysis in brown adipocytes; Authors - Qingwen Zhao
human	WB	Oncotarget; The platelet isoform of phosphofructokinase contributes to metabolic reprogramming and maintains cell proliferation in clear cell renal cell carcinoma.; Authors - Jun Wang