PFKL Monoklonaler Antikörper

PFKL Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1H2G2

Kat-Nr. : 68385-1-Ig

Synonyme

1H2G2, 6-phosphofructokinase type B, ATP-dependent 6-phosphofructokinase, liver type, ATP-PFK, EC:2.7.1.11

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  WB analysis using 68385-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68385-1-Ig (PFKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IHC staining of human lung cancer using 68385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 68385-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 68385-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 68385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 68385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 68385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 68385-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HuH-7 using 68385-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HuH-7 using 68385-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HEK-293 using 68385-1-Ig

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug PFKL Monoclonal antibody (68385-1-Ig, Clone:1H2G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Lebergewebe, humanes Ovarialkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HuH-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

68385-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA PFKL und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PFKL fusion protein Ag8250
• Vollständiger Name: phosphofructokinase, liver
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC008964
• Gene symbol: PFKL
• Gene ID (NCBI): 5211
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFKL (6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings, PFK exists as a system of three subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs (PMID: 2139864). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	BMC Cardiovasc Disord; Identification of hub glycolysis-related genes in acute myocardial infarction and their correlation with immune infiltration using bioinformatics analysis; Authors - Xiaoqing Zhang
mouse	WB	Metabolism; Protein kinase N1 deficiency results in upregulation of cerebral energy metabolism and is highly protective in in vivo and in vitro stroke models; Authors - Stephanie Zur Nedden
human	IHC	Redox Biol; Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation; Authors - Jiayan Huang