PFTK1 Polyklonaler Antikörper

PFTK1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21612-1-AP

Synonyme

CDK14, hPFTAIRE1, KIAA0834, PFTAIRE protein kinase 1, PFTAIRE1, PFTK1

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  WB analysis of HeLa using 21612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 21612-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21612-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21612-1-AP (PFTK1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21612-1-AP (PFTK1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

21612-1-AP bindet in WB, IHC, IF, ELISA PFTK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PFTK1 fusion protein Ag16259
• Vollständiger Name: PFTAIRE protein kinase 1
• Berechnetes Molekulargewicht: 469 aa, 53 kDa
• Beobachtetes Molekulargewicht: 48-53 kDa
• GenBank-Zugangsnummer: BC136477
• Gene symbol: PFTK1
• Gene ID (NCBI): 5218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFTK1 is a Cdc2-related protein kinase, also named as PFTAIRE1, CDK14 and belongs to the CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It acts as a cyclin-dependent kinases that regulates cell cycle progression and cell proliferation. The PFTK1 protein shows high homology with mouse Pftaire1, a Cdc2-related protein expressed primarily in the postnatal and adult nervous system(PMID:9202329). The protein contains 2 predicted nuclear localization signals that the N-terminal nuclear localization signals do not play a role in vivo(PMID:11313143). It has 3 isoforms produced by alternative splicing. This antibody is specific to PFTK1.

Publikationen

Species	Application	Title
human	WB	Commun Biol; Methylation-modulated PFTK1 regulates gefitinib resistance via Wnt/β-catenin signaling in EGFR mutant non-small-cell lung cancer cells; Authors - Xiaoting Jia
human	WB	Mol Ther Oncolytics; Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus; Authors - Dakota W. Goad
mouse	WB, IHC, IF	Toxicol Lett; CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.; Authors - Daniel Pollack
human	WB	World Neurosurg; MiR-205 Inhibits Sporadic Vestibular Schwannoma Cell Proliferation by Targeting Cyclin-Dependent Kinase 14.; Authors - Xiaoling Yin
human	WB,IHC	Biomed Pharmacother; MiR-542-3p, a microRNA targeting CDK14, suppresses cell proliferation, invasiveness, and tumorigenesis of epithelial ovarian cancer.; Authors - Jingwei Li