Pepsinogen I Polyklonaler Antikörper

Pepsinogen I Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 17330-1-AP

Synonyme

PGA5, PGI, Pepsinogen-5, Pepsin A-5, Pepsin A

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• 

  WB analysis using 17330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17330-1-AP (Pepsinogen I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 17330-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17330-1-AP (Pepsinogen I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse stomach using 17330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17330-1-AP (Pepsinogen I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 17330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17330-1-AP (Pepsinogen I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BGC-823-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

17330-1-AP bindet in WB, IHC, IF, ELISA Pepsinogen I und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Pepsinogen I fusion protein Ag11309
• Vollständiger Name: pepsinogen 5, group I (pepsinogen A)
• Berechnetes Molekulargewicht: 388 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC029055
• Gene symbol: PGA5
• Gene ID (NCBI): 5222
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pepsinogen I belongs to the peptidase A1 family. It shows particularly broad specificity and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The gene encodes a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. This antibody can recognize PGA3(pepsinogen 3), PGA4(pepsinogen 4), PGA5(pepsinogen 5).

Publikationen

Species	Application	Title
		J Pathol; Bmi1 marks gastric stem cells located in the isthmus in mice.; Authors - Takuto Yoshioka
mouse	WB	Biochem Biophys Res Commun; Changed PGA and POSTN levels in choroid plexus are associated with depressive-like behaviors in mice.; Authors - Jie Liu
rat	IF	PLoS One; The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival.; Authors - Pål Vange
human	IHC	Hum Pathol; Clinical Significance of Spasmolytic Polypeptide-expressing Metaplasia and Intestinal Metaplasia in EBV-associated and EBV-negative Gastric Cancer.; Authors - Yu Zhang
mouse	IHC	Dev Dyn; Glypican-6 and Glypican-4 stimulate embryonic stomach growth by regulating Hedgehog and non-canonical Wnt signaling; Authors - Wen Shi
pig	IHC	J Agric Food Chem; Casein Hydrolysate Enhances Upper Gastrointestinal Chemosensing and Gastric Acid Secretion in Pigs; Authors - Chunlong Mu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

FAISAL (Verified Customer) (05-03-2021)

I found this antibody very good and give very good and consistent result in low concentration.

• Applications: Western Blot, Immunohistochemistry
• Cell Tissue Type: cell and tissue