HPGDS Polyklonaler Antikörper

HPGDS Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 22522-1-AP

Synonyme

Glutathione S transferase, GST class sigma, GSTS, H PGDS, HPGDS, PGDS, Prostaglandin H2 D isomerase, PTGDS2

---

Galerie der Validierungsdaten

• 

  WB analysis using 22522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22522-1-AP (HPGDS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 22522-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22522-1-AP (HPGDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22522-1-AP (HPGDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22522-1-AP (HPGDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22522-1-AP (HPGDS antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 22522-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22522-1-AP (HPGDS antibody at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenmilzgewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

22522-1-AP bindet in WB, IHC, IF, ELISA HPGDS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HPGDS fusion protein Ag18290
• Vollständiger Name: prostaglandin D2 synthase, hematopoietic
• Berechnetes Molekulargewicht: 199 aa, 23 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC020734
• Gene symbol: PGDS
• Gene ID (NCBI): 27306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hematopoietic prostaglandin D synthase (HPGDS) is expressed in human Th2 lymphocytes, antigen-presenting cells, mast cells and microglia. HPGDS converts PGH2 into PGD2, a mediator thought to play a pivotal role in airway allergy and inflammatory processes (PMID: 16624958). HPGDS deficiency is a critical factor that impedes cutaneous wound healing in diabetes. Overexpressing Hpgds in ADSCs could be an effective way to accelerate DW healing by alleviating inflammatory cells recruitment and increasing M2 polarization in the proliferation phase (PMID: 35922870). HPGDS is a cytosolic homodimer of 26 kDa subunits which reveal a well-defined active site, enabling a structure-based design of inhibitors (PMID: 24900177).

Publikationen

Species	Application	Title
mouse	IF	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
human	WB	EMBO Mol Med; Tranilast directly targets NLRP3 to treat inflammasome-driven diseases.; Authors - Yi Huang
mouse	WB	Molecules; Integrative Analysis of Proteomics and Metabolism Reveals the Potential Roles of Arachidonic Acid Metabolism in Hypoxia Response in Mouse Spleen; Authors - Yujing Guo