PGM2L1 Polyklonaler Antikörper

PGM2L1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13942-1-AP

Synonyme

BM32A, EC:2.7.1.106, Glucose 1,6-bisphosphate synthase, Phosphoglucomutase-2-like 1, PMMLP

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  WB analysis using 13942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13942-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 13942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13942-1-AP

  IP result of anti-PGM2L1 (IP:13942-1-AP, 4ug; Detection:13942-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human gliomas using 13942-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13942-1-AP (PGM2L1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13942-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13942-1-AP (PGM2L1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

13942-1-AP bindet in WB, IHC, IP, ELISA PGM2L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGM2L1 fusion protein Ag5019
• Vollständiger Name: phosphoglucomutase 2-like 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC059360
• Gene symbol: PGM2L1
• Gene ID (NCBI): 283209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGM2L1 (Phosphoglucomutase 2 Like 1) is an enzyme-like protein involved in carbohydrate metabolism. PGM2L1 catalyzes the formation of G16BP by using the glycolytic intermediate 1,3-bisphosphoglycerate as a phosphate donor and glucose-1-phosphate or glucose-6-phosphate as a phosphate acceptor. PGM2L1 plays a crucial role in glycogen metabolism, glycolysis, and gluconeogenesis as a key enzyme, and increasing evidence links its function to cancer metabolism and progression. While PGM2 has a wide tissue distribution, PGM2L1 is highly expressed in the brain, accounting for the elevated concentrations of glucose-1,6-bisphosphate found there (PMID: 33979636, PMID: 40027181).

Publikationen

Species	Application	Title
human	WB,IHC	Mol Metab; Glucose-1,6-bisphosphate: a new gatekeeper of cerebral mitochondrial pyruvate uptake; Authors - Motahareh Solina Safari ; KD Validated
mouse	WB	Metabolism; Protein kinase N1 deficiency results in upregulation of cerebral energy metabolism and is highly protective in in vivo and in vitro stroke models; Authors - Stephanie Zur Nedden
human	WB,IHC	Cell Death Dis; Cancer-associated fibroblasts secrete CSF3 to promote TNBC progression via enhancing PGM2L1-dependent glycolysis reprogramming; Authors - Wenqi Qin