PGP Polyklonaler Antikörper

PGP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 25081-1-AP

Synonyme

Aspartate-based ubiquitous Mg(2+)-dependent phosphatase, AUM, EC:3.1.3.21, EC:3.1.3.48, G3PP



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, K-562-Zellen, Mausherzgewebe, SH-SY5Y-Zellen, Y79-Zellen
Erfolgreiche IPY79-Zellen
Erfolgreiche Detektion in IHCMausherzgewebe, humanes Skelettmuskelgewebe, humanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen
Erfolgreiche Detektion in FC (Intra)SH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

25081-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA PGP und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PGP fusion protein Ag18623
Vollständiger Name phosphoglycolate phosphatase
Berechnetes Molekulargewicht 321 aa, 34 kDa
Beobachtetes Molekulargewicht 34 kDa
GenBank-ZugangsnummerBC157035
Gene symbol PGP
Gene ID (NCBI) 283871
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGP(phosphoglycolate phosphatase) belongs to the HAD-like hydrolase superfamily. It is an essential intermediate in the biosynthetic pathway of cardiolipin and a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle. PGP catalyzes the formation of phosphatidylglycerol from phosphatidylglycerophosphate.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PGP antibody 25081-1-APProtokoll herunterladen
IHC protocol for PGP antibody 25081-1-APProtokoll herunterladenl
IF protocol for PGP antibody 25081-1-APProtokoll herunterladen
IP protocol for PGP antibody 25081-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Acta Biomater

Systematic evaluation of multifunctional paclitaxel-loaded polymeric mixed micelles as a potential anticancer remedy to overcome multidrug resistance.

Authors - Jiulong Zhang
humanIHC

Front Cell Dev Biol

Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.

Authors - Lingyun Zhang
humanWB

Chin Med

The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters

Authors - Wenjie Huang
humanWB,IF

J Nanobiotechnology

Combination of polythyleneimine regulating autophagy prodrug and Mdr1 siRNA for tumor multidrug resistance

Authors - Changduo Wang
humanWB

Eur J Med Chem

Design, synthesis and biological evaluation of novel phenylfuran-bisamide derivatives as P-glycoprotein inhibitors against multidrug resistance in MCF-7/ADR cell

Authors - Zhikun Yang
ratIF

J Peripher Nerv Syst

Effect of age on metabolomic changes in a model of paclitaxel-induced peripheral neurotoxicity

Authors - Roberta Bonomo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kerstin (Verified Customer) (10-11-2024)

nice detection of PGP in HT1080 WT cells compared to KO cells, few unspecific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HT1080
PGP Antibody Western Blot validation (1:5000 dilution) in HT1080 (Cat no:25081-1-AP)
FH

Yu Siong (Verified Customer) (10-13-2019)

3 protein bands at 150 kDa are of rat liver whole tissue lysate.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rat liver tissue
PGP Antibody Western Blot, validation (1:500 dilution) in Rat liver tissue (Cat no:25081-1-AP)