PHF12 Polyklonaler Antikörper

PHF12 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 24485-1-AP

Synonyme

KIAA1523, PF1, PHD factor 1, PHD finger protein 12, PHF12

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  WB analysis of mouse brain using 24485-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24485-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 24485-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 24485-1-AP

  IP result of anti-PHF12 (IP:24485-1-AP, 4ug; Detection:24485-1-AP 1:500) with mouse brain tissue lysate 3600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mauslungengewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

24485-1-AP bindet in WB, IP, ELISA PHF12 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHF12 fusion protein Ag18471
• Vollständiger Name: PHD finger protein 12
• Berechnetes Molekulargewicht: 1004 aa, 110 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC121043
• Gene symbol: PHF12
• Gene ID (NCBI): 57649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plant homeodomain [PHD] zinc finger protein 12 (PHF12), also known Pf1, is a member of the PHD zinc finger family of proteins. Pf1 associates with a chromatin-interacting protein complex comprised of MRG15, Sin3B, and histone deacetylase 1 (HDAC1) that functions as a transcriptional modulator.

Publikationen

Species	Application	Title
human	WB	EMBO Rep; MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.; Authors - Gongwei Wu
human	WB	J Transl Med; PHF12 regulates HDAC1 to promote tumorigenesis via EGFR/AKT signaling pathway in non-small cell lung cancer; Authors - Yiru Kong ; KD Validated