PHF6 Polyklonaler Antikörper

PHF6 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 51062-1-AP

Synonyme

BORJ, CENP-31, PHD-like zinc finger protein, PHF 6

---

• 

  WB analysis using 51062-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51062-1-AP (PHF6 antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 51062-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 51062-1-AP (PHF6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 51062-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF6 antibody (51062-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Zervixkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Magenkrebsgewebe, Maushirngewebe, Maus-Magengewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

51062-1-AP bindet in WB, IHC, IF/ICC, ELISA PHF6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHF6 fusion protein Ag2102
• Vollständiger Name: PHD finger protein 6
• Berechnetes Molekulargewicht: 365aa,41 kDa; 312aa,35 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC005994
• Gene symbol: PHF6
• Gene ID (NCBI): 84295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHF6 protein is highly conserved in vertebrates. Structurally, PHF6 protein contains two plant homeodomain (PHD)-like zinc fingers, two nuclear localization sequences and a nucleolar localization sequence, suggesting that PHF6 may play a role in the regulation of transcription. PHD zinc fingers are structurally conserved modules that interact with chromatin or mediate protein-protein interactions. PHF6 associates with subunits of the PAF1 transcription elongation complex including PAF1, LEO1, CTR9 and CDC73.The interaction of PHF6 with the PAF1 complex plays a critical role in PHF6 function in neuronal migration in the cerebral cortex.

Publikationen

Species	Application	Title
human	WB	Leuk Res; MiR-1306-5p promotes cell proliferation and inhibits cell apoptosis in acute myeloid leukemia by downregulating PHF6 expression.; Authors - Xiang Gao