PHLDA1 Polyklonaler Antikörper

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• KD/KO Validated

PHLDA1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 18263-1-AP

Synonyme

Apoptosis-associated nuclear protein, DT1P1B11, PHRIP, Pleckstrin homology-like domain family A member 1, PQ-rich protein

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  WB analysis using 18263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18263-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 18263-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18263-1-AP (PHLDA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A375 using 18263-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using PHLDA1 antibody (18263-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, BxPC-3-Zellen, HeLa-Zellen, MDA-MB-231-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

18263-1-AP bindet in WB, IHC, IF/ICC, ELISA PHLDA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHLDA1 fusion protein Ag13125
• Vollständiger Name: pleckstrin homology-like domain, family A, member 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC018929
• Gene symbol: PHLDA1
• Gene ID (NCBI): 22822
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHLDA1, also known as PHRIP and TDAG51, is a multifunctional protein involved in various biological processes. It can induce apoptosis in various cell types, including T cells, hippocampal cells, endothelial cells, melanoma cells, and mouse embryonic fibroblasts(PMID: 30207029). PHLDA1 plays a role in inhibiting growth factor signaling and has been implicated in tumor suppression through its ability to repress Akt activity by binding to phosphatidylinositol (PIP) lipids(PMID: 36142223).

Publikationen

Species	Application	Title
mouse	WB	Brain Behav Immun; PHLDA1 promotes microglia-mediated neuroinflammation via regulating K63-linked ubiquitination of TRAF6.; Authors - Chaojun Han ; KD Validated
mouse,human	WB,IHC	Inflammation; Multiple Machine Learning Identifies Key Gene PHLDA1 Suppressing NAFLD Progression; Authors - Zhenwei Yang
rat	WB	Life Sci; PHLDA1 is a new therapeutic target of oxidative stress and ischemia reperfusion-induced myocardial injury.; Authors - Yuxuan Guo ; KD Validated
mouse	WB	Inflammation; TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT-GSK-3β Pathway.; Authors - Chuntian Liu ; KD Validated
mouse	WB	Hum Exp Toxicol; Loss of PHLDA1 has a protective role in OGD/R-injured neurons via regulation of the GSK-3β/Nrf2 pathway.; Authors - F Yang ; KD Validated
mouse	WB	Neuroreport; Egr1 promotes Nlrc4-dependent neuronal pyroptosis through phlda1 in an in-vitro model of intracerebral hemorrhage; Authors - Jian Wang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lukas (Verified Customer) (01-31-2025)

worked very well for Western blot in patient primary fibroblasts and cell lines (in 1% milk at +4°C overnight)

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human dermal fibroblasts, HeLa, hTERT-RPE1

PHLDA1 Antibody Western Blot validation (1:2000 dilution) in human dermal fibroblasts, HeLa, hTERT-RPE1 (Cat no:18263-1-AP)