PI3 Kinase p110 Alpha Polyklonaler Antikörper

PI3 Kinase p110 Alpha Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 27921-1-AP

Synonyme

p110 alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PIK3CA, PtdIns 3 kinase subunit alpha

---

• 

  WB analysis using 27921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• CoIP: See 1 publications below

Produktinformation

27921-1-AP bindet in WB, CoIP, ELISA PI3 Kinase p110 Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p110 Alpha fusion protein Ag27539
• Vollständiger Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Berechnetes Molekulargewicht: 1068 aa, 124 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC113601
• Gene symbol: PIK3CA
• Gene ID (NCBI): 5290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publikationen

Species	Application	Title
rat	WB	J Ethnopharmacol; Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology.; Authors - Shi Dong
mouse,human	WB	Heliyon; Anticancer potential of grifolin in lung cancer treatment through PI3K/AKT pathway inhibition; Authors - Li Wang
mouse	WB	JCI Insight; Regulatory T-cell and neutrophil extracellular traps interaction contributes to the development of immunosuppression in sepsis; Authors - Yuxin Shi
human	WB	Anticancer Drugs; STK214947, a novel indole alkaloids, inhibits HeLa and SK-HEP-1 cells survival and EMT process by blocking the Notch3 and Akt signals; Authors - Zihan Xu
human	WB	J Immunother; Vitexin Inhibits TNBC Progression and Metastasis by Modulating Macrophage Polarization Through EGFR Signaling; Authors - Yufeng Lin
mouse	WB	Eur J Pharmacol; Novel Applications of Metformin in the Treatment of Septic Myocardial Injury Based on Metabolomics and Network Pharmacology; Authors - Xingyu Li