PI3 Kinase p110 Beta Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PI3 Kinase p110 Beta Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (8)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

343

Rezensionen (1)

Kat-Nr. : 20584-1-AP

Synonyme

p110 BETA, PI3 Kinase p110 beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

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Galerie der Validierungsdaten

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  WB analysis of rat liver using 20584-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 20584-1-AP (PI3 Kinase p110 Beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 20584-1-AP

  IP Result of anti-PI3K p110(beta) (IP:20584-1-AP, 4ug; Detection:20584-1-AP 1:300) with HepG2 cells lysate 2700ug.

• 

  IHC staining of human lung cancer using 20584-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20584-1-AP (PI3 Kinase p110 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20584-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20584-1-AP (PI3 Kinase p110 Beta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 20584-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PI3 Kinase p110 Beta (20584-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 17 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

20584-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA PI3 Kinase p110 Beta und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, Hausschwein, Huhn, Hund, Kaninchen, Maus, Ratte, Zebrafisch, Riesenscheibenbrasse (Megalobrama amblycephala)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: phosphoinositide-3-kinase, catalytic, beta polypeptide
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: NM_006219
• Gene symbol: PIK3CB
• Gene ID (NCBI): 5291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.

Publikationen

Species	Application	Title
human		Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
mouse		Redox Biol; GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux; Authors - Xiaoxiao Ji
human		Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng
human	IHC	Clin Cancer Res; STAT3/HOTAIR signaling axis regulates HNSCC growth in an EZH2-dependent manner.; Authors - Shanshan Sun
human,rabbit		Environ Res; Effects of external environment on promoter methylation of PIK3R5 and related pathway regulation in steroid-induced femoral head necrosis; Authors - Wentao Qin
mouse		Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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