PI3 Kinase p55 Gamma Polyklonaler Antikörper

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• KD/KO Validated

PI3 Kinase p55 Gamma Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 27035-1-AP

Synonyme

p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 27035-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27035-1-AP (PI 3 Kinase p55 gamma antibody at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293 using 27035-1-AP

  WB result of PI 3 Kinase p55 gamma antibody (27035-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI 3 Kinase p55 gamma transfected HEK-293 cells.

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  WB analysis of HUVEC using 27035-1-AP

  WB result of PIK3R3 antibody (27035-1-AP) on whole cell extract from HUVEC targeted by shRNAs (#1, #2) or a control shRNA (NT). (Data from Dr. Fuqiang Geng, Weill Cornell Medical College).

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  IHC staining of human kidney using 27035-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 27035-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 27035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 27035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen, HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maushodengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

27035-1-AP bindet in WB, IHC, ELISA PI3 Kinase p55 Gamma und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p55 Gamma fusion protein Ag25884
• Vollständiger Name: phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC021622
• Gene symbol: PIK3R3
• Gene ID (NCBI): 8503
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.

Publikationen

Species	Application	Title
human	IHC	Am J Physiol Lung Cell Mol Physiol; p55PIK Deficiency and its N-terminal Derivative inhibit Inflammation and Emphysema in COPD mouse model.; Authors - Ting Wang
human,mouse	WB,IHC	BMC Cancer; Circ_0021350 plays an oncogene role by regulating miR-1207-3p/PIK3R3 in glioblastoma; Authors - Cheng Tan ; KD Validated