PIN1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

PIN1 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

5N20

Kat-Nr. : 81857-1-RR

Synonyme

Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8, 5N20

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  WB analysis of HeLa using 81857-1-RR

  WB result of PIN1 antibody (81857-1-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIN1 transfected HeLa cells.

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  WB analysis using 81857-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81857-1-RR (PIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 81857-1-RR

  IP result of anti-PIN1 (IP:81857-1-RR, 4ug; Detection:81857-1-RR 1:20000) with HepG2 cells lysate 1920 ug.

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  IP experiment of NIH/3T3 using 81857-1-RR

  IP result of anti-PIN1 (IP:81857-1-RR, 4ug; Detection:81857-1-RR 1:20000) with NIH/3T3 cells lysate 1120 ug.

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  IHC staining of human pancreas cancer using 81857-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81857-1-RR (PIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 81857-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81857-1-RR (PIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 81857-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (81857-1-RR, Clone: 5N20 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 81857-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81857-1-RR against Human PIN1 were performed. The affinity constant is 0.17 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81857-1-RR bindet in WB, IHC, IF/ICC, IP, ELISA PIN1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PIN1 fusion protein Ag0767
• Vollständiger Name: peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC002899
• Gene symbol: PIN1
• Gene ID (NCBI): 5300
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).