PIPPIN Polyklonaler Antikörper

PIPPIN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25013-1-AP

Synonyme

CSDC2, RNA-binding protein PIPPin, RNA binding protein PIPPin, Cold shock domain-containing protein C2

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  WB analysis of mouse brain using 25013-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25013-1-AP (PIPPIN antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25013-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25013-1-AP (PIPPIN antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 25013-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25013-1-AP (PIPPIN antibody at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25013-1-AP bindet in WB, IHC, ELISA PIPPIN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIPPIN fusion protein Ag16216
• Vollständiger Name: cold shock domain containing C2, RNA binding
• Berechnetes Molekulargewicht: 153 aa, 17 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC067113
• Gene symbol: CSDC2
• Gene ID (NCBI): 27254
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIPPIN, also known as CSDC2 (Cold shock domain-containing protein C2) is an RNA‐binding protein (RBP), highly enriched in the rat brain, specifically enriched in some pyramidal neurons of the cerebral cortex and in the Purkinje cells of the cerebellum (PMID: 10446180). PIPPIN has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Nuclear CSDC2 is connected to proliferation and cytoplasmic CSDC2 to terminal differentiation in the decidua and that CSDC2 could regulate differentiation during decidua development (PMID: 30078185, PMID: 17053029).