PISD Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PISD Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 16401-1-AP

Synonyme

PSSC, PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain

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  WB analysis of K-562 using 16401-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16401-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 16401-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16401-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16401-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16401-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16401-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 16401-1-AP

  IP result of anti-PISD (IP:16401-1-AP, 3ug; Detection:16401-1-AP 1:500) with L02 cells lysate 1400ug.

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  IHC staining of human kidney using 16401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HepG2-Zellen, humanes Nierengewebe, humanes Milzgewebe, K-562-Zellen, L02-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

16401-1-AP bindet in WB, IHC, IP, ELISA PISD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PISD fusion protein Ag9499
• Vollständiger Name: phosphatidylserine decarboxylase
• Berechnetes Molekulargewicht: 375aa,43 kDa; 408aa,46 kDa
• Beobachtetes Molekulargewicht: 45~55 kDa
• GenBank-Zugangsnummer: BC001482
• Gene symbol: PISD
• Gene ID (NCBI): 23761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.This antibody is specific to PISD. The sequence correspond to the beta isoform.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; Aberrant ER-mitochondria communication is a common pathomechanism in mitochondrial disease; Authors - Patricia Morcillo
human	WB	Cancer Lett; LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.; Authors - Yawen Ma
human		Front Cell Dev Biol; Screening and diagnosis of triple negative breast cancer based on rapid metabolic fingerprinting by conductive polymer spray ionization mass spectrometry and machine learning; Authors - Yaoyao Song
human	WB	J Clin Biochem Nutr; Metabolic changes induced by TGF-β1 via reduced expression of phosphatidylserine decarboxylase during myofibroblast transition; Authors - Nobuhiko Uchida
mouse	WB	bioRxiv; Distinct yet Overlapping Functions of VMP1 and TMEM41B in Modulating Hepatic Lipoprotein Secretion and Autophagy in MASH; Authors - Allen Chen
human	WB	Cell Stem Cell; The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.; Authors - Ayesh K Seneviratne ; KO Validated