PITPNB Polyklonaler Antikörper

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• KD/KO Validated

PITPNB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13110-1-AP

Synonyme

PI TP beta, PITPNB, PtdIns transfer protein beta, PtdInsTP, PtdInsTP beta, VIB1B

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  WB analysis of mouse lung using 13110-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13110-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13110-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 13110-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13110-1-AP (PITPNB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13110-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13110-1-AP (PITPNB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 13110-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13110-1-AP (PITPNB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13110-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13110-1-AP (PITPNB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, Mauslungengewebe, Raji-Zellen, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below

Produktinformation

13110-1-AP bindet in WB, IHC, ELISA PITPNB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PITPNB fusion protein Ag3774
• Vollständiger Name: phosphatidylinositol transfer protein, beta
• Berechnetes Molekulargewicht: 272 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC031427
• Gene symbol: PITPNB
• Gene ID (NCBI): 23760
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Virol; Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.; Authors - Hongliang Wang ; KO Validated
human	WB	Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta ; KO Validated