PITRM1 Polyklonaler Antikörper

PITRM1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10101-2-AP

Synonyme

hMP1, hPreP, KIAA1104, Metalloprotease 1, MP1, pitrilysin metallopeptidase 1, Pitrilysin metalloproteinase 1, PITRM1, PreP

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 10101-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10101-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10101-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 10101-2-AP

  IP Result of anti-PITRM1 (IP:10101-2-AP, 4ug; Detection:10101-2-AP 1:500) with A549 cells lysate 2000ug.

• 

  IHC staining of human breast cancer using 10101-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10101-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 10101-2-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 10101-2-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 10101-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 10101-2-AP (PITRM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, A549-Zellen, Maushodengewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Osteosarkomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10101-2-AP bindet in WB, IP, IHC, IF, ELISA PITRM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PITRM1 fusion protein Ag0150
• Vollständiger Name: pitrilysin metallopeptidase 1
• Berechnetes Molekulargewicht: 60 kDa, 117 kDa
• Beobachtetes Molekulargewicht: 110-117 kDa
• GenBank-Zugangsnummer: BC001150
• Gene symbol: PITRM1
• Gene ID (NCBI): 10531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.This antibody is specific to PITRM1.

Publikationen

Species	Application	Title
human	WB	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
mouse	WB	PLoS One; Proteolysis controls endogenous substance p levels.; Authors - Mitchell Andrew J AJ