PITX1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PITX1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10873-1-AP

Synonyme

BFT, Homeobox protein PITX1, paired like homeodomain 1, Pituitary homeobox 1, PITX1, POTX, PTX1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10873-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10873-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10873-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10873-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10873-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10873-1-AP

  IP Result of anti-PITX1 (IP:10873-1-AP, 4ug; Detection:10873-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IP experiment of HeLa using 10873-1-AP

  IP Result of anti-PITX1 (IP:10873-1-AP, 4ug; Detection:10873-1-AP 1:800) with HeLa cells lysate 1200ug.

• 

  IHC staining of human tonsillitis using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10873-1-AP

  Immunofluorescent analysis of HepG2 cells, using PITX1 antibody 10873-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

10873-1-AP bindet in WB, IP, IHC, IF, ELISA PITX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PITX1 fusion protein Ag1303
• Vollständiger Name: paired-like homeodomain 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC009412
• Gene symbol: PITX1
• Gene ID (NCBI): 5307
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PITX1, also named as Pituitary homeobox 1 or BFT, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. Bicoid subfamily. PITX1 localizes in the nucleus and may play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
	WB,IF	Hum Mutat; Mandibular-pelvic-patellar syndrome is a novel PITX1-related disorder due to alteration of PITX1 transactivation ability.; Authors - Godelieve Morel
mouse	WB	Endocrinology; Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice.; Authors - Hiraku Kameda
human	WB,IHC	Int J Biochem Cell Biol; Paired like homeodomain 1 and SAM and SH3 domain-containing 1 in the progression and prognosis of head and neck squamous cell carcinoma.; Authors - Yu Jin ; KD Validated
rat	WB,IF	Toxicol Lett; All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression.; Authors - Wang Yun-Guo YG