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PKM2-specific Monoklonaler Antikörper
PKM2-specific Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
1C11C7
Kat-Nr. : 60268-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293-Zellen, 4T1-Zellen, C6-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, ROS1728-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen, HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:30000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:100-1:1000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 6 publications below |
WB | See 63 publications below |
IHC | See 12 publications below |
IF | See 27 publications below |
IP | See 8 publications below |
CoIP | See 6 publications below |
Produktinformation
60268-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PKM2-specific und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | PKM pyruvate kinase, muscle |
Berechnetes Molekulargewicht | 58 kDa |
Beobachtetes Molekulargewicht | 58 kDa |
GenBank-Zugangsnummer | NM_002654 |
Gene symbol | PKM |
Gene ID (NCBI) | 5315 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for PKM2-specific antibody 60268-1-Ig | Protokoll herunterladen |
IHC protocol for PKM2-specific antibody 60268-1-Ig | Protokoll herunterladenl |
IF protocol for PKM2-specific antibody 60268-1-Ig | Protokoll herunterladen |
IP protocol for PKM2-specific antibody 60268-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Adv Mater Dual-Targeted Novel Temozolomide Nanocapsules Encapsulating siPKM2 Inhibit Aerobic Glycolysis to Sensitize Glioblastoma to Chemotherapy | ||
Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation | ||
Nat Commun MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration | ||
Mol Cell The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis. | ||
Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.
| ||
J Exp Clin Cancer Res NONO interacts with nuclear PKM2 and directs histone H3 phosphorylation to promote triple-negative breast cancer metastasis |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tianyi (Verified Customer) (01-12-2024) | Antigen retrieval in TE buffer, and incubated with secondary antibody AF647 at room temperature for 2 h.
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