PKC Gamma Polyklonaler Antikörper

PKC Gamma Polyklonal Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Kat-Nr. : CL647-14364

Synonyme

PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14

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Galerie der Validierungsdaten

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  WB analysis using CL647-14364

  Various lysates were subjected to SDS PAGE followed by western blot with CL647-14364 (PKC Gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-14364 bindet in WB PKC Gamma und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKC Gamma fusion protein Ag5681
• Vollständiger Name: protein kinase C, gamma
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 76-80 kDa
• GenBank-Zugangsnummer: BC047876
• Gene symbol: PRKCG
• Gene ID (NCBI): 5582
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.