PKC Alpha Monoklonaler Antikörper

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• KD/KO Validated

PKC Alpha Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1D11F4

Kat-Nr. : 66421-1-Ig

Synonyme

PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13

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  WB analysis using 66421-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66421-1-Ig

  WB result of PKC Alpha antibody (66421-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Alpha transfected HEK-293 cells.

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  WB analysis of HeLa using 66421-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66421-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66421-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66421-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66421-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66421-1-Ig (PKC alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66421-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66421-1-Ig (PKC alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66421-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Alpha antibody (66421-1-Ig, Clone: 1D11F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66421-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Alpha antibody (66421-1-Ig, Clone: 1D11F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 66421-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66421-1-Ig(PKC alpha antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66421-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (66421-1-Ig, Clone:1D11F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66421-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA PKC Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PKC Alpha fusion protein Ag17275
• Vollständiger Name: protein kinase C, alpha
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC109274
• Gene symbol: PKC Alpha
• Gene ID (NCBI): 5578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Publikationen

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Arsenic interferes with spermatogenesis involving Rictor/mTORC2-mediated blood-testis barrier disruption in mice; Authors - Xiujuan Li
human	WB	Life Sci; Transcription factor PRRX1-activated ANXA6 facilitates EGFR-PKCα complex formation and enhances cisplatin sensitivity in bladder cancer; Authors - Jinlong Cao