PKLR Polyklonaler Antikörper

PKLR Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17580-1-AP

Synonyme

EC:2.7.1.40, PK1, PKL, Pyruvate kinase isozymes L/R, Pyruvate kinase PKLR

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  WB analysis of K-562 using 17580-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17580-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17580-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 17580-1-AP

  IP result of anti-PKLR (IP:17580-1-AP, 4ug; Detection:17580-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of human liver using 17580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 17580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Mausnierengewebe, Mauslebergewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

17580-1-AP bindet in WB, IHC, IP, ELISA PKLR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKLR fusion protein Ag11096
• Vollständiger Name: pyruvate kinase, liver and RBC
• Berechnetes Molekulargewicht: 574 aa, 62 kDa, 585 aa, 63 kDa
• Beobachtetes Molekulargewicht: 58-62 kDa
• GenBank-Zugangsnummer: BC025737
• Gene symbol: PKLR
• Gene ID (NCBI): 5313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1; Authors - Yanling Zhang
human	WB	ACS Pharmacol Transl Sci; All Blood Brain Barrier Cell Types Demonstrate Capability to Influence Differential Tenofovir and Emtricitabine Metabolism and Transport in the Brain; Authors - Hannah N Wilkins