PKM Polyklonaler Antikörper

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PKM Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 25659-1-AP

Synonyme

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2, PK-M2

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  WB analysis of KEK293 cells using 25659-1-AP

  WB result of PKM antibody (25659-1-AP, 1:3000) with si-control and si-PKM transfected HEK293 cell.

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  WB analysis using 25659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25659-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25659-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25659-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 25659-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25659-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 25659-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human normal colon using 25659-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25659-1-AP (PKM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 25659-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (25659-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 25659-1-AP

  Immunofluorescent analysis of HeLa cells using 25659-1-AP (PKM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HEK-293-Zellen, KEK293-Zellen, MCF-7-Zellen, Maushirngewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen, Rattenhirngewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

25659-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PKM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKM fusion protein Ag20270
• Vollständiger Name: PKM pyruvate kinase, muscle
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC000481
• Gene symbol: PKM
• Gene ID (NCBI): 5315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823). This antibody can recognize both PK-M1 and PK-M2.

Publikationen

Species	Application	Title
human	WB,IP	EMBO Rep; Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.; Authors - Guang Yang
human	WB	Oncogene; HMGB1/SET/HAT1 complex-mediated SASH1 repression drives glycolysis and metastasis in lung adenocarcinoma; Authors - Fan Kou
human	WB,IP,CoIP	Int J Mol Sci; Acetyltransferase P300 Regulates Glucose Metabolic Reprogramming through Catalyzing Succinylation in Lung Cancer; Authors - Qingzhi Ma
human	WB	Cell Mol Biol Lett; N6-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing; Authors - Anqi Wang
mouse	CoIP	CNS Neurosci Ther; Novel Insights Into DLAT's Role in Alzheimer's Disease-Related Copper Toxicity Through Microglial Exosome Dynamics; Authors - Xiang Ma
human	WB	Cell Commun Signal; Spinster homolog 2 (SPNS2) deficiency drives endothelial cell senescence and vascular aging via promoting pyruvate metabolism mediated mitochondrial dysfunction; Authors - Haojun Tang