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  • KD/KO Validated

PKM1-specific Polyklonaler Antikörper

PKM1-specific Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(92)

Kat-Nr. : 15821-1-AP

Synonyme

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, HepG2/MCF-7-Zellen, Maus-Kolongewebe, Mausherzgewebe, Maus-Muskel-/-Lebergewebe, Maus-Skelettmuskelgewebe, Multizellen/-gewebe, Rattenhirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Gliomgewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Mausherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15821-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA PKM1-specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name PKM pyruvate kinase, muscle
Berechnetes Molekulargewicht 58 kDa
Beobachtetes Molekulargewicht 58 kDa
GenBank-ZugangsnummerNM_182470
Gene symbol PKM
Gene ID (NCBI) 5315
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PKM1-specific antibody 15821-1-APProtokoll herunterladen
IHC protocol for PKM1-specific antibody 15821-1-APProtokoll herunterladenl
IP protocol for PKM1-specific antibody 15821-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseWB,IHC

Nature

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.

Authors - Christofk Heather R HR
  • KD Validated
human

Nat Immunol

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Authors - Di Huang
humanWB

Sci Transl Med

ATP citrate lyase drives vascular remodeling in systemic and pulmonary vascular diseases through metabolic and epigenetic changes

Authors - Yann Grobs
humanWB,CoIP

Nat Commun

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence

Authors - Yinsheng Wu
humanWB

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang
humanWB

Nat Commun

The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.

Authors - Fang Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Swati (Verified Customer) (09-26-2025)

The antibody functions perfectly with no background. Clear band observed at 58kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 in 5% milk in TBS-T
  • Cell Tissue Type: Human lung pericytes
FH

Pallavi (Verified Customer) (03-31-2025)

I used 1:5000 anti-PKM1 15821-1-AP, it worked perfectly. The reviews were quite helpful to decide which antibody to buy. The delivery was on time. Just make sure the delivery company hands over the package to you. so better to keep a track of the package.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: purified protein