PKM Polyklonaler Antikörper

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• KD/KO Validated

PKM Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 10078-2-AP

Synonyme

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 cells using 10078-2-AP

  WB result of PKM antibody (10078-2-AP, 1:1000) with si-Control and si-PKM transfected HEK293 cells.

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  WB analysis of multi-cells/tissue using 10078-2-AP

  WB result of 10078-2-AP (PKM antibody) with various lysates.

• 

  WB analysis using 10078-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 10078-2-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10078-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10078-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10078-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10078-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10078-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10078-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10078-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10078-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10078-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10078-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10078-2-AP (PKM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10078-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (10078-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10078-2-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKM (10078-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Mausherzgewebe, Maushirngewebe, Mausmuskelgewebe, MCF-7-Zellen, Multizellen/-gewebe, NIH/3T3-Zellen, Raji-Zellen, Ratten-Skelettmuskelgewebe, SH-SY5Y-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

10078-2-AP bindet in WB, IHC, IF, FC, ELISA PKM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKM fusion protein Ag0110
• Vollständiger Name: PKM pyruvate kinase, muscle
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC000481
• Gene symbol: PKM
• Gene ID (NCBI): 5315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKM, also named as OIP3, PK2, PK3, PKM1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PKM1; however, the primary isoform from four independent tumours is PKM2.(PMID:18337823). This antibody can recognize both PKM1 and PKM2.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
pig	WB	Br J Pharmacol; PMID: 30515768; Authors - Xiaoying Lian
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
pig	WB	Sci Rep; Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.; Authors - Liangliang Fu
human	IF	Int J Biol Sci; Dissection of Targeting Molecular Mechanisms of Aristolochic Acid-induced Nephrotoxicity via a Combined Deconvolution Strategy of Chemoproteomics and Metabolomics.; Authors - Qian Zhang
mouse	WB	Am J Physiol Endocrinol Metab; PMID: 31211620; Authors - Yang Yang