PKM2-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PKM2-specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(271)

Rezensionen (5)

Kat-Nr. : 15822-1-AP

Synonyme

PKM2, PK-M2, PKM2 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

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  WB analysis of HEK-293 cells using 15822-1-AP

  WB result of PKM2 specific antibody (15822-1-AP, 1:5000) with si-control and si-PKM2 transfected HEK293 cells.

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  WB analysis using 15822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. Negative control: mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue.

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  WB analysis using 15822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15822-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15822-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 15822-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of 293 cell using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control.).

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  WB analysis using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).

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  WB analysis of Recombinant protein using 15822-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15822-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 15822-1-AP

  IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:4000) with A549 cells lysate 920 ug.

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  IP experiment of MCF-7 using 15822-1-AP

  IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:2000) with MCF-7 cells lysate 1880 ug.

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  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15822-1-AP (PKM2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15822-1-AP (PKM2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 15822-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15822-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, C6-Zellen, HEK-293-Zellen, HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen, Maus-Magengewebe
• Erfolgreiche IP: A549-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 18 publications below
• WB: See 244 publications below
• IHC: See 43 publications below
• IF: See 42 publications below
• IP: See 12 publications below
• CoIP: See 8 publications below
• ChIP: See 2 publications below

Produktinformation

15822-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA PKM2-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PKM pyruvate kinase, muscle
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_002654
• Gene symbol: PKM
• Gene ID (NCBI): 5315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform. This antibody can detect PKM2 monomer, dimer and tetramer (PMID: 36196894).

Publikationen

Species	Application	Title
mouse,human	WB,IHC	Nature; The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.; Authors - Christofk Heather R HR ; KD Validated
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human	WB	Mol Cell; p53 induces circFRMD4A to suppress cancer development through glycolytic reprogramming and cuproptosis; Authors - Quan Liao
human	WB,IP	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
human	WB	Mol Cell; A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.; Authors - Jin-Zhou Huang
mouse,human	WB,IHC	Biomaterials; PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy; Authors - Lu Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Swati (Verified Customer) (09-26-2025)

Shows a clear band around 58 kDa. No non specific bands observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1: 1000 in 5% milk in TBS-T
• Cell Tissue Type: Human lung pericytes

FH

Pallavi (Verified Customer) (03-31-2025)

i used 1:5000 anti-PKM2 15522-1-AP for western blot on purified protein and it worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: purified protein

FH

Shinford (Verified Customer) (09-24-2023)

The antibody concentration of 1:1000 worked well, and bands could be detected in a variety of cell lines. There were slight non-specific bands in some tissue and organ cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Tumor cell lines derived from multiple systems, tissues and organs。

PKM2-specific Antibody Western Blot validation (1:1000 dilution) in Tumor cell lines derived from multiple systems, tissues and organs。 (Cat no:15822-1-AP)

FH

Kristian (Verified Customer) (06-07-2021)

PKM2 (red) is detected in proximale tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:15822-1-AP)

FH

Kishor (Verified Customer) (02-22-2019)

I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

PKM2-specific Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:15822-1-AP)