PKM2-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PKM2-specific Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

161

Rezensionen (3)

Kat-Nr. : 15822-1-AP

Synonyme

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PK-M2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 cells using 15822-1-AP

  WB result of PKM2 specific antibody (15822-1-AP, 1:5000) with si-control and si-PKM2 transfected HEK293 cells.

• 

  WB analysis of MCF-7 using 15822-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15822-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15822-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 15822-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of hela cells using 15822-1-AP

  WB result of 15822-1-AP (PKM2 antibody). (anti-PKM2 and PKLR antibodies are used as negative controls.).

• 

  WB analysis of hela cells using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ).

• 

  WB analysis of mouse brain tissue using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.).

• 

  WB analysis of 293 cell using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control.).

• 

  WB analysis using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).

• 

  WB analysis of Recombinant protein using 15822-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15822-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 15822-1-AP

  IP result of anti-PKM2-specific(IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:4000) with A549 cells lysate 920 ug.

• 

  IP experiment of MCF-7 using 15822-1-AP

  IP result of anti-PKM2-specific(IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:2000) with MCF-7 cells lysate 1880 ug.

• 

  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15822-1-AP (PKM2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15822-1-AP (PKM2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 15822-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PKM2 antibody 19060-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 15822-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Maus-Magengewebe, MCF-7-Zellen
• Erfolgreiche IP: A549-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 14 publications below
• WB: See 147 publications below
• IHC: See 30 publications below
• IF: See 26 publications below
• IP: See 6 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

15822-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA PKM2-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PKM pyruvate kinase, muscle
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_002654
• Gene symbol: PKM
• Gene ID (NCBI): 5315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Nature; The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.; Authors - Christofk Heather R HR ; KD Validated
human	WB,IP	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
human	WB	Mol Cell; A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.; Authors - Jin-Zhou Huang
human,mouse	WB,IHC	Biomaterials; PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy; Authors - Lu Wang
human	IP	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB	Clin Transl Med; USP7 promotes non-small-cell lung cancer cell glycolysis and survival by stabilizing and activating c-Abl; Authors - Yuanming He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (09-24-2023)

The antibody concentration of 1:1000 worked well, and bands could be detected in a variety of cell lines. There were slight non-specific bands in some tissue and organ cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Tumor cell lines derived from multiple systems, tissues and organs。

PKM2-specific Antibody Western Blot validation (1:1000 dilution) in Tumor cell lines derived from multiple systems, tissues and organs。 (Cat no:15822-1-AP)

FH

Kristian (Verified Customer) (06-07-2021)

PKM2 (red) is detected in proximale tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:15822-1-AP)

FH

Kishor (Verified Customer) (02-22-2019)

I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

PKM2-specific Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:15822-1-AP)

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