PLA2G12A Polyklonaler Antikörper

PLA2G12A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 16009-1-AP

Synonyme

phospholipase A2, group XIIA, Phosphatidylcholine 2-acylhydrolase 12A, GXII sPLA2, GXII, FKSG38

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  WB analysis of mouse skeletal muscle using 16009-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16009-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16009-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16009-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16009-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16009-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 16009-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 16009-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 16009-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 16009-1-AP (PLA2G12A Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 16009-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 16009-1-AP (PLA2G12A Antibody) at dilution of 1:50.

• 

  IF Staining of HEK-293 using 16009-1-AP

  Immunofluorescent analysis of HEK-293 cells using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HEK-293-Zellen, Mausherzgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

16009-1-AP bindet in WB, IHC, IF/ICC, ELISA PLA2G12A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLA2G12A fusion protein Ag8722
• Vollständiger Name: phospholipase A2, group XIIA
• Berechnetes Molekulargewicht: 189 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21-24 kDa
• GenBank-Zugangsnummer: BC017218
• Gene symbol: PLA2G12A
• Gene ID (NCBI): 81579
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLA2G12A (or PLA2G12) gene encodes group XII secreted phospholipase A2 (sPLA2) which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Cellular arachidonate (AA) release and prostaglandin (PG) production is regulated by sPLA(2)s, groups III and XII. Group XII sPLA2 have relatively low specific activity and are structurally and functionally distinct from other sPLA2s. Cells transfected with group XII sPLA(2) exhibited abnormal morphology, suggesting a unique functional aspect of this enzyme. Role of sPLA2s as potential tumour biomarkers and therapeutic targets for various cancers have been reported.

Publikationen

Species	Application	Title
human	IHC	Int J Cardiol; Phospholipase A2 expression in coronary thrombus is increased in patients with recurrent cardiac events after acute myocardial infarction.; Authors - Takahashi Soichiro S
mouse	IHC,WB	Atherosclerosis; Quantitative trait locus mapping in mice identifies phospholipase Pla2g12a as novel atherosclerosis modifier.; Authors - Alexandros Nicolaou
rat	WB	Exp Ther Med; Therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease.; Authors - Tao Li
human	IHC	J Atheroscler Thromb; The Expression of Groups IIE and V Phospholipase A2 is Associated with an Increased Expression of Osteogenic Molecules in Human Calcified Aortic Valves.; Authors - Koji Suzuki
human,mouse	WB	Nat Commun; Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis; Authors - Xiaoqiong Wei