PLA2G1B Polyklonaler Antikörper

PLA2G1B Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15843-1-AP

Synonyme

PLA2, Phosphatidylcholine 2-acylhydrolase 1B, EC:3.1.1.4

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  WB analysis using 15843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15843-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 15843-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human pancreas cancer using 15843-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15843-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 15843-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using PLA2G1B antibody (15843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, Mausmilzgewebe, Hausschwein-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

15843-1-AP bindet in WB, IHC, IF-P, ELISA PLA2G1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLA2G1B fusion protein Ag8603
• Vollständiger Name: phospholipase A2, group IB (pancreas)
• Berechnetes Molekulargewicht: 70aa,8 kDa; 148aa,16 kDa
• Beobachtetes Molekulargewicht: 13-16 kDa
• GenBank-Zugangsnummer: BC005386
• Gene symbol: PLA2G1B
• Gene ID (NCBI): 5319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.

Publikationen

Species	Application	Title
mouse	WB	Sci Rep; Identification and verification of biomarkers associated with arachidonic acid metabolism in non-alcoholic fatty liver disease; Authors - Jia Li
mouse	WB	Food Chem; Dietary sn-2 palmitate influences cognitive behavior by increasing the transport of liver-produced lysophosphatidylcholine VLCPUFAs to the brain; Authors - Teng Wei