PLA2G4A Monoklonaler Antikörper

PLA2G4A Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2D10H8

Kat-Nr. : 68133-1-Ig

Synonyme

2D10H8, cPLA2, cPLA2 alpha, Lysophospholipase, Phosphatidylcholine 2-acylhydrolase

---

• 

  WB analysis using 68133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 68133-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68133-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 68133-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 68133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 68133-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLA2G4A antibody (68133-1-Ig, Clone: 2D10H8 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

68133-1-Ig bindet in WB, IHC, IF/ICC, ELISA PLA2G4A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLA2G4A fusion protein Ag32674
• Vollständiger Name: phospholipase A2, group IVA (cytosolic, calcium-dependent)
• Berechnetes Molekulargewicht: 749 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa
• GenBank-Zugangsnummer: BC114340
• Gene symbol: PLA2G4A
• Gene ID (NCBI): 5321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).

Publikationen

Species	Application	Title
rat	WB	J Nat Med; Insight of action mechanism of Astragaloside IV for relieving of cerebral ischemic injury in a rat model of middle cerebral artery occlusion reperfusion via proteomics and network pharmacology; Authors - Xin-Hua Zhu
human	WB	Cancer Cell Int; CENPF interaction with PLA2G4A promotes glioma growth by modulating mTORC1 and NF-κB pathways; Authors - Junhong Li
human	IHC	J Transl Med; scRNA-seq reveals that VEGF signaling mediates the response to neoadjuvant anlotinib combined with PD-1 blockade therapy in non-small cell lung cancer; Authors - Ziqi Huang
human	WB	FASEB J; Mechano-induced arachidonic acid metabolism promotes keratinocyte proliferation through cPLA2 activity regulation; Authors - Shengzhou Shan