PLAC8 Polyklonaler Antikörper

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• KD/KO Validated

PLAC8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12284-1-AP

Synonyme

BM-004, C15, onzin, placenta 8, Placenta-specific gene 8 protein

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  WB analysis of human peripheral blood leukocyte using 12284-1-AP

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 12284-1-AP (PLAC8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12284-1-AP (PLAC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12284-1-AP (PLAC8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12284-1-AP (PLAC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12284-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12284-1-AP (PLAC8 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood leukocyte, PANC-1 cells
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hirngewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

12284-1-AP bindet in WB, IHC, IF, CoIP, ELISA PLAC8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLAC8 fusion protein Ag2939
• Vollständiger Name: placenta-specific 8
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC012205
• Gene symbol: PLAC8
• Gene ID (NCBI): 51316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLAC8, also named as Protein C15, is a placenta-specific protein. The expression was downregulated upon activation, particularly in dendritic cells generated in vitro from CD34 (142230) progenitor cells. The MW of PLAC8 is about 12-20kd.

Publikationen

Species	Application	Title
human	IHC	FASEB J; PLAC8 promotes the autophagic activity and improves the growth priority of human trophoblast cells.; Authors - Xuan Feng
human	IHC	Int J Med Sci; Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray.; Authors - Jianfeng Gan
human	WB,IHC	Histochem Cell Biol; PLAC8 contributes to the malignant behaviors of cervical cancer cells by activating the SOX4-mediated AKT pathway; Authors - Boya Deng ; KD Validated
human	IF	PLoS Genet; Single-cell RNA profiling reveals classification and characteristics of mononuclear phagocytes in colorectal cancer; Authors - Tiantian Ji
human	WB,IHC	Front Immunol; Identification and validation of immune and diagnostic biomarkers for interstitial cystitis/painful bladder syndrome by integrating bioinformatics and machine-learning; Authors - Tao Zhou
human,mouse	WB,CoIP	Commun Biol; PLAC8 attenuates pulmonary fibrosis and inhibits apoptosis of alveolar epithelial cells via facilitating autophagy; Authors - Wei Sun