PLCB3 Polyklonaler Antikörper

PLCB3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51139-1-AP

Synonyme

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3

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  WB analysis of HeLa using 51139-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 51139-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 51139-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MDA-MB-231 using 51139-1-AP

  IP result of anti-PLCB3 (IP:51139-1-AP, 4ug; Detection:51139-1-AP 1:1000) with MDA-MB-231 cells lysate 1280 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

51139-1-AP bindet in WB, IP, ELISA PLCB3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: phospholipase C, beta 3 (phosphatidylinositol-specific)
• Berechnetes Molekulargewicht: 1234 aa, 139 kDa
• Beobachtetes Molekulargewicht: 139 kDa
• GenBank-Zugangsnummer: BC142681
• Gene symbol: PLCB3
• Gene ID (NCBI): 5331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.