PLEK Monoklonaler Antikörper

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• KD/KO Validated

PLEK Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D5C2

Kat-Nr. : 66431-1-Ig

Synonyme

P47, Platelet 47 kDa protein, pleckstrin, PLEK

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Galerie der Validierungsdaten

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  WB analysis of U-937 using 66431-1-Ig

  WB result of PLEK antibody (66431-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells.

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  WB analysis of HL-60 using 66431-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66431-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 66431-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 66431-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66431-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66431-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 66431-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse spleen using 66431-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Hausschwein-Milzgewebe, THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66431-1-Ig bindet in WB, IHC, IF, ELISA PLEK und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLEK fusion protein Ag3187
• Vollständiger Name: pleckstrin
• Berechnetes Molekulargewicht: 350 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-47 kDa
• GenBank-Zugangsnummer: BC018549
• Gene symbol: PLEK
• Gene ID (NCBI): 5341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)