PLEKHF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PLEKHF1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20389-1-AP

Synonyme

Apoptosis inducing protein, APPD, LAPF, Phafin 1, PHAFIN1, PLEKHF1, ZFYVE15

---

Galerie der Validierungsdaten

• 

  WB analysis of human skeletal muscle using 20389-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 20389-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20389-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 20389-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20389-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 20389-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 20389-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20389-1-AP (PLEKHF1 Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 20389-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20389-1-AP (PLEKHF1 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 20389-1-AP

  Immunofluorescent analysis of HepG2 cells, using PLEKHF1 antibody 20389-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, humanes Nierengewebe, Mauslungengewebe, Mausmilzgewebe, Maus-Skelettmuskelgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

20389-1-AP bindet in WB, IHC, IF, ELISA PLEKHF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLEKHF1 fusion protein Ag14228
• Vollständiger Name: pleckstrin homology domain containing, family F (with FYVE domain) member 1
• Berechnetes Molekulargewicht: 279 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC002744
• Gene symbol: PLEKHF1
• Gene ID (NCBI): 79156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLEKHF1, also named as APPD, LAPF, and ZFYVE15, is a 279 amino acid protein, which contains one PH domain and one FYVE-type zinc finger. PLEKHF1 translocates to lysosome during apoptosis, localizing in the nucleus and cytoplsam. PLEKHF1 is highly expressed in heart and skeletal muscle and weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung. PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. PLEKHF1 modulates the protein content of the plasma membrane and was involved in different endocytosis events.

Publikationen

Species	Application	Title
human	WB	Breast Cancer Res; Functional characterization of the 19q12 amplicon in grade III breast cancers.; Authors - Natrajan Rachael R ; KD Validated
mouse	WB,IF	Sci China Life Sci; Local administration of liposomal-based Plekhf1 gene therapy attenuates pulmonary fibrosis by modulating macrophage polarization; Authors - Lifeng Yan ; KD Validated