PLEKHM1 Polyklonaler Antikörper

PLEKHM1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16202-1-AP

Synonyme

162 kDa adapter protein, AP162, B2, KIAA0356, OPTB6, PLEKHM1

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Galerie der Validierungsdaten

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  WB analysis of ROS1728 using 16202-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16202-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ROS1728-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16202-1-AP bindet in WB, IHC, ELISA PLEKHM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLEKHM1 fusion protein Ag9145
• Vollständiger Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC064361
• Gene symbol: PLEKHM1
• Gene ID (NCBI): 9842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	WB	Nutrients; Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.; Authors - Hongkun Lin