PLEKHM1 Polyklonaler Antikörper

PLEKHM1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16202-1-AP

Synonyme

AP162, OPTB6, PH domain-containing family M member 1, Pleckstrin homology domain-containing family M member 1

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  WB analysis of ROS1728 using 16202-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16202-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ROS1728-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16202-1-AP bindet in WB, IHC, ELISA PLEKHM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLEKHM1 fusion protein Ag9145
• Vollständiger Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC064361
• Gene symbol: PLEKHM1
• Gene ID (NCBI): 9842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLEKHM1, also known as AP162, OPTB6, belongs to the Pleckstrin homology domain protein family. PLEKHM1 functions as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events within the endolysosomal system, thereby coupling autophagic and endocytic trafficking pathways. In osteoclasts, PLEKHM1 is crucial for bone resorption by regulating the vesicle transport process and ensuring the formation of the ruffled border.

Publikationen

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	WB	Nutrients; Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.; Authors - Hongkun Lin
		Cell Biol Toxicol; Downregulation of MYBL1 in endothelial cells contributes to atherosclerosis by repressing PLEKHM1-inducing autophagy; Authors - Shi-Ao Ding