Plasminogen Monoklonaler Antikörper

Plasminogen Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1F6A11

Kat-Nr. : 66399-1-Ig

Synonyme

PLG, 1F6A11, Angiostatin, EC:3.4.21.7, Plasmin heavy chain A, short form

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  WB analysis of human plasma using 66399-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66399-1-Ig (PLG Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66399-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66399-1-Ig (PLG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66399-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66399-1-Ig (PLG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 66399-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66399-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66399-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

66399-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA Plasminogen und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Plasminogen fusion protein Ag9938
• Vollständiger Name: plasminogen
• Berechnetes Molekulargewicht: 810 aa, 91 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC060513
• Gene symbol: Plasminogen
• Gene ID (NCBI): 5340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

Publikationen

Species	Application	Title
mouse	WB	Angiogenesis; PDGFD switches on stem cell endothelial commitment.; Authors - Weisi Lu
human	WB	Aging (Albany NY); NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.; Authors - Pingping Ma
mouse	WB	Front Cell Dev Biol; Elevated Levels of miR-144-3p Induce Cholinergic Degeneration by Impairing the Maturation of NGF in Alzheimer's Disease.; Authors - Lan-Ting Zhou
mouse	IHC	Biomed Pharmacother; Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia.; Authors - Lina Wei
human	WB,IHC	Clin Transl Oncol; A prognostic gene signature for gastric cancer and the immune infiltration-associated mechanism underlying the signature gene, PLG; Authors - Hui Shi
human	WB	Mol Cancer; Single-cell multi-omics reveals that FABP1 + renal cell carcinoma drive tumor angiogenesis through the PLG-PLAT axis under fatty acid reprogramming; Authors - Yiqiu Wang