PLK1 Polyklonaler Antikörper

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• KD/KO Validated

PLK1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 10305-1-AP

Synonyme

PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

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Galerie der Validierungsdaten

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  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat stomach using 10305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 10305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 10305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 10305-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 10305-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 10305-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLK1 (10305-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushodengewebe, Rattenhodengewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Ratten-Magengewebe, Maus-Magengewebe, Rattenlebergewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

10305-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PLK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLK1 fusion protein Ag0284
• Vollständiger Name: polo-like kinase 1 (Drosophila)
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC002369
• Gene symbol: PLK1
• Gene ID (NCBI): 5347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

Publikationen

Species	Application	Title
human	WB,IF	J Clin Invest; PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models; Authors - Marta Baldrighi
human	WB	Adv Sci (Weinh); Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer; Authors - Huaiyuan Liang ; KD Validated
human,mouse	WB	Sci Adv; Cascade dynamic assembly/disassembly of DNA nanoframework enabling the controlled delivery of CRISPR-Cas9 system; Authors - Nachuan Song
human	WB,IF	Nat Commun; Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer.; Authors - Wengui Shi
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
rat	WB	Cell Rep; Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex; Authors - Kellen D Winden

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011