PLOD2 Polyklonaler Antikörper

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• KD/KO Validated

PLOD2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(53)

Rezensionen (1)

Kat-Nr. : 21214-1-AP

Synonyme

PLOD2-Specific, EC:1.14.11.4, Lysyl hydroxylase 2, PLOD2/LH2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

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  WB analysis of A431 using 21214-1-AP

  WB result of PLOD2 antibody (21214-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2-Specific transfected A431 cells.

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  WB analysis of THLE-2 using 21214-1-AP

  WB result of PLOD2 antibody (21214-1-AP 1:3000; room temperature for 1.5 hours) with wild-type and PLOD2 knockout THLE-2 cells.

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  WB analysis using 21214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21214-1-AP

  DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 21214-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 21214-1-AP

  IP result of anti-PLOD2 (IP:21214-1-AP, 4ug; Detection:21214-1-AP 1:500) with mouse testis tissue lysate 5000ug.

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  IHC staining of human liver cancer using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21214-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD2 antibody (21214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 21214-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLOD2 (21214-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, A431-Zellen, DU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HT-1080.Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Pankreasgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 11 publications below
• WB: See 37 publications below
• IHC: See 21 publications below
• IF: See 7 publications below

Produktinformation

21214-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA PLOD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLOD2 fusion protein Ag15614
• Vollständiger Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
• Berechnetes Molekulargewicht: 758 aa, 85 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC037169
• Gene symbol: PLOD2
• Gene ID (NCBI): 5352
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

Publikationen

Species	Application	Title
human	WB,IHC	J Clin Invest; Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.; Authors - Yulong Chen
zebrafish	WB	Cancer Res; Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis; Authors - Ying Liu
mouse,human	WB	Cancer Discov; Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis.; Authors - Eisinger-Mathason T S Karin TS
mouse	WB	Arthritis Rheumatol; Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis.; Authors - Akio Yamamoto
human	WB	Proc Natl Acad Sci U S A; Intratumoral oxygen gradients mediate sarcoma cell invasion.; Authors - Daniel M Lewis
mouse	IHC	Arthritis Rheum; Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis.; Authors - Remst D F G DF

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RICHA (Verified Customer) (12-02-2019)

Excellent

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast