PLOD3 Polyklonaler Antikörper

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• KD/KO Validated

PLOD3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 11027-1-AP

Synonyme

LH3, Lysyl hydroxylase 3, PLOD3

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Galerie der Validierungsdaten

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  WB analysis using 11027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 11027-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11027-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11027-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11027-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11027-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11027-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11027-1-AP

  IP Result of anti-PLOD3 (IP:11027-1-AP, 3ug; Detection:11027-1-AP 1:500) with HepG2 cells lysate 6000ug.

• 

  IHC staining of human pancreas cancer using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11027-1-AP

  Immunofluorescent analysis of HepG2 cells using 11027-1-AP (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, Maus-Plazenta-Gewebe, PC-3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Produktinformation

11027-1-AP bindet in WB, IP, IHC, IF, ELISA PLOD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLOD3 fusion protein Ag1480
• Vollständiger Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• Berechnetes Molekulargewicht: 738 aa, 85 kDa
• Beobachtetes Molekulargewicht: 80-85 kDa
• GenBank-Zugangsnummer: BC011674
• Gene symbol: PLOD3
• Gene ID (NCBI): 8985
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Publikationen

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
		Nat Commun; Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.; Authors - Luigi Scietti
human	WB,IF	Nat Commun; Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.; Authors - Blerida Banushi
human	WB	Am J Hum Genet; A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene.; Authors - Salo Antti M AM
mouse	WB	PLoS Genet; Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta.; Authors - Wayne A Cabral
human	WB	Matrix Biol; Mutations in PLOD3, encoding lysyl hydroxylase 3, cause a complex connective tissue disorder including recessive dystrophic epidermolysis bullosa-like blistering phenotype with abnormal anchoring fibrils and type VII collagen deficiency.; Authors - Hassan Vahidnezhad

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