PLRG1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PLRG1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11914-1-AP

Synonyme

Pleiotropic regulator 1, PLRG1, PRL1

---

• 

  WB analysis of K-562 using 11914-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11914-1-AP

  WB result of PLRG1 antibody (11914-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLRG1 transfected HEK-293 cells.

• 

  WB analysis of BxPC-3 using 11914-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11914-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11914-1-AP

  IP result of anti-PLRG1 (IP:11914-1-AP, 4ug; Detection:11914-1-AP 1:1000) with HEK-293 cells lysate 1400ug.

• 

  IHC staining of human brain using 11914-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 11914-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, BxPC-3-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11914-1-AP bindet in WB, IP, IHC, ELISA PLRG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLRG1 fusion protein Ag2527
• Vollständiger Name: pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
• Berechnetes Molekulargewicht: 514 aa, 57 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC020786
• Gene symbol: PLRG1
• Gene ID (NCBI): 5356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
human	WB	Cell Death Differ; USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.; Authors - Shuyan Liu